Effect of chicken infectious bronchitis vaccine on morphogenesis and differentiation of cells in caecal tonsils

The study of the chickens’ immune system morphofunctional state allows assessing critical periods their development and body as a whole, well effectiveness vaccine prevention methods. purpose this was to identify morphological immunohistochemical changes in caecal tonsils chickens aged 8, 20, 40, 90, 110 days for infectious bronchitis. During study, following research methods were used: cytological, histological, immunohistochemical, morphometric, light-optical, statistical. Histological preparations poultry vaccinated unvaccinated groups analysed studied. Up 20 age, no lymphoid nodules detected chickens, both non-vaccinated groups. morphometric studies conducted, which showed that vaccination accelerates formations early stages postnatal period ontogenesis, especially days, is manifested by an increase number size relative control group. Immunohistochemical have established placement accumulation T-lymphocytes with markers CD4+ , CD8+ B-lymphocytes (CD20+ ) CD45RA+ cells tonsils, namely diffuse cells, depend on age multiplicity vaccinations. It proved 8 T-lymphocyte subpopulations surface prevailed over CD20+ . With immunizations (after three-fold against bronchitis chickens), immunocytometric indicated 40 90 there clear mature 1.58 1.37 times, respectively. Considering fact lymphocytes 1.49 times greater than led sharp decrease immunoregulatory index must be factored when carrying out preventive vaccinations